Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Purification of post-transcriptionally modified tRNAs for enhanced cell-free translation systems.

Nucleic acids research·2026
Same author

CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning.

BMC biotechnology·2026
Same author

Synthetic cells by the numbers.

iScience·2025
Same author

One-pot cloning and protein expression platform for genetic engineering.

bioRxiv : the preprint server for biology·2025
Same author

High Yield, Low Magnesium Flexizyme Reactions in a Water-Ice Eutectic Phase.

Biochemistry·2025
Same author

Purification of post-transcriptionally modified tRNAs for enhanced cell-free translation systems.

bioRxiv : the preprint server for biology·2025
Same journal

Optimized tRNA structure-seq reveals robust tRNA secondary structures in <i>S. cerevisiae</i> under mild stress conditions.

RNA (New York, N.Y.)·2026
Same journal

SERIPH: A Two-Step Extraction Protocol for Selective Enrichment of Semi-Extractable RNAs.

RNA (New York, N.Y.)·2026
Same journal

Reduced Sensitivity to RNA Structural Differences Distinguishes Eukaryotic Pus4 from Bacterial TruB.

RNA (New York, N.Y.)·2026
Same journal

Puf3 contributes to changes in mRNA solubility, translation elongation dynamics at rare arginine codons and loss of protein homeostasis in cells lacking Not4.

RNA (New York, N.Y.)·2026
Same journal

RBM38 Regulates HORMAD1 Splicing to Enhances MEK Inhibitor Sensitivity in Breast Cancer.

RNA (New York, N.Y.)·2026
Same journal

EF-P Inhibits Ribosomal α-Hydroxy Acid Incorporation: Strategic tRNA Body Selection for Co-incorporating α-Hydroxy Acids and Nonproteinogenic Amino Acids into Depsipeptides.

RNA (New York, N.Y.)·2026
See all related articles

Related Experiment Video

Updated: Dec 20, 2025

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.7K

Highly specific, multiplexed isothermal pathogen detection with fluorescent aptamer readout.

Lauren M Aufdembrink1, Pavana Khan1, Nathaniel J Gaut2

  • 1Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA.

RNA (New York, N.Y.)
|June 3, 2020
PubMed
Summary
This summary is machine-generated.

A new method called Aptamer-based Nucleic Acid Sequence Amplification (Apta-NASBA) prevents false positives in cell-free pathogen detection. This advance enables sensitive, multiplexed detection using various equipment, including low-cost platforms for field use.

Keywords:
NASBAT7 RNA polymerasefluorescent aptamerisothermal amplificationpathogen detection

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.6K
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.6K

Related Experiment Videos

Last Updated: Dec 20, 2025

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.7K
Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.6K
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.6K

Area of Science:

  • Synthetic biology
  • Molecular diagnostics
  • Biotechnology

Background:

  • Cell-free systems offer simplified pathogen detection compared to live-cell systems.
  • Nucleic acid sequence based amplification (NASBA) is a known cell-free amplification technique.
  • Cell-free systems are prone to false positives due to non-specific reactions.

Purpose of the Study:

  • To develop a method to suppress false positives in cell-free amplification systems.
  • To enable sensitive and multiplexed detection of nucleic acids using fluorescent aptamers.
  • To create a versatile detection platform compatible with various equipment.

Main Methods:

  • Inclusion of a promoter-less, amplicon-unassociated DNA duplex to inhibit false positives.
  • Development of Aptamer-based Nucleic Acid Sequence Amplification (Apta-NASBA) using fluorescent aptamers as tags.
  • Testing Apta-NASBA with a fluorescence microplate reader, qPCR instrument, and a Raspberry Pi-based 3D-printed platform.

Main Results:

  • Apta-NASBA effectively suppresses false positives in cell-free reactions.
  • Achieved a detection limit of 1 pM for nucleic acid targets.
  • Demonstrated multiplexed, multicolor fluorescent readout capabilities.
  • Confirmed compatibility with diverse detection equipment, including a low-cost field-deployable platform.

Conclusions:

  • Apta-NASBA provides a robust solution for accurate cell-free pathogen detection.
  • The method offers high sensitivity and multiplexing capabilities.
  • Its compatibility with various platforms, including low-cost options, facilitates field applications.