GFF Utilities: GffRead and GffCompare
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Updated: Dec 19, 2025

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
Published on: September 7, 2022
Geo Pertea1,2, Mihaela Pertea1,2
1Center for Computational Biology, Johns Hopkins University, Baltimore, MD, 21218, USA.
Abstract:
GTF (Gene Transfer Format) and GFF (General Feature Format) are popular file formats used by bioinformatics programs to represent and exchange information about various genomic features, such as gene and transcript locations and structure. GffRead and GffCompare are open source programs that provide extensive and efficient solutions to manipulate files in a GTF or GFF format. While GffRead can convert, sort, filter, transform, or cluster genomic features, GffCompare can be used to compare and merge different gene annotations.
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