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Related Experiment Video

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Cell barrier characterization in transwell inserts by electrical impedance spectroscopy.

Georg Linz1, Suzana Djeljadini1, Lea Steinbeck1

  • 1DWI - Leibniz Institute for Interactive Materials, Forckenbeckstr. 50, 52074 Aachen, Germany; RWTH Aachen University, Aachener Verfahrenstechnik-Chemical Process Engineering, Forckenbeckstrasse 51, 52074, Aachen, Germany.

Biosensors & Bioelectronics
|June 10, 2020
PubMed
Summary

A new electrical impedance spectroscopy (EIS) method uses a simple electrode to test cell barrier integrity. This technique reveals both transepithelial electrical resistance (TEER) and cell layer capacitance, offering deeper insights into barrier function.

Keywords:
Caco-2Electrical impedance spectroscopy (EIS)Membrane capacitanceSonoporationTransepithelial electrical resistance (TEER)Transwell insert

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Biophysics

Background:

  • Cell barrier integrity is crucial for physiological function and drug development.
  • Traditional methods like transepithelial electrical resistance (TEER) offer limited information.
  • A need exists for more comprehensive and accessible cell barrier assessment techniques.

Purpose of the Study:

  • To introduce and validate a novel impedance-based method for cell barrier integrity testing.
  • To demonstrate the capability of electrical impedance spectroscopy (EIS) combined with a simple electrode for monitoring cell barriers.
  • To compare the new method's findings with established TEER measurements and analyze distinct barrier properties.

Main Methods:

  • Utilized a four-electrode electrical impedance spectroscopy (EIS) setup with a commercial chopstick-like electrode (STX-1) connected to a potentiostat.
  • Monitored cell barriers (Caco-2 monolayer) cultivated in transwell inserts.
  • Performed electric circuit modeling to determine capacitive properties alongside TEER, analyzing responses to membrane coatings, permeability enhancers (EGTA, saponin), and sonoporation.

Main Results:

  • The new EIS method successfully measured cell barrier properties in transwell inserts.
  • TEER values from the new protocol correlated with standard methods across different membrane coatings.
  • Cell layer capacitance revealed distinct patterns related to surface materials and was significantly altered by saponin, indicating independent information from TEER.

Conclusions:

  • The described impedance-based method provides a simple yet powerful tool for assessing cell barrier integrity.
  • Combining TEER and capacitance measurements offers a more complete characterization of cell monolayers.
  • This accessible technique using commercial electrodes has the potential to become a standard in cell barrier research.