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Plasma membrane H+ -ATPases-mediated cytosolic proton gradient regulates pollen tube growth
Wei Chen1, Peng-Fei Jia1, Wei-Cai Yang1,2
1State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing, 100101, China.
Abstract:
The polar growth of pollen tubes is essential for the delivery of sperm cells during fertilization in angiosperms. How this polar growth is regulated has been a long-standing question. An in vitro pharmacological assay previously implicated proton flux in pollen tube growth, although genetic and cellular supporting evidence was lacking. Here, we report that protons form a gradient from the pollen tube tip to the shank region and this gradient is generated by three members of Arabidopsis H+ -ATPases (AHAs). Genetic analysis suggested that these AHAs are essential for pollen tube growth, thus providing new insight into the regulation of polar growth.
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