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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Related Experiment Video

Updated: Dec 18, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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Dual-Mode Superresolution Imaging Using Charge Transfer Dynamics in Semiconducting Polymer Dots.

Yifei Jiang1,2, Qiongzheng Hu2, Haobin Chen2

  • 1Department of Chemistry, Clemson University, Clemson, SC, 29634, USA.

Angewandte Chemie (International Ed. in English)
|June 11, 2020
PubMed
Summary

Researchers developed novel semiconducting polymer dots exhibiting spontaneous fluorescence switching. These dots enable superresolution imaging with <10 nm precision in spontaneous mode and ~1 nm precision in photoactivated mode for enhanced biological structure visualization.

Keywords:
fluorescent probesphotoactivationphotoswitchingpolymerssuperresolution imaging

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Area of Science:

  • Nanotechnology
  • Biophysics
  • Materials Science

Background:

  • Conjugated polymer-based single-particle heterojunctions exhibit stochastic fluctuations.
  • Photogenerated hole population dynamics influence fluorescence switching behavior.

Purpose of the Study:

  • To develop novel semiconducting polymer dots for superresolution imaging.
  • To leverage spontaneous fluorescence switching and photoactivation for enhanced imaging capabilities.

Main Methods:

  • Utilizing 405 nm irradiation to induce charge recombination and activate single-particle emission.
  • Developing semiconducting polymer dots capable of operating in dual superresolution imaging modes.
  • Demonstrating superresolution imaging of biological structures like microtubules and clathrin-coated pits.

Main Results:

  • Developed semiconducting polymer dots with spontaneous fluorescence switching.
  • Achieved <10 nm localization precision in spontaneous switching mode for large-area imaging.
  • Attained ~1 nm localization precision in photoactivation/deactivation mode for high-resolution imaging of small structures.

Conclusions:

  • The novel polymer dots offer versatile superresolution imaging options.
  • Spontaneous switching mode provides efficient large-area imaging with high precision.
  • Photoactivation/deactivation mode offers superior resolution for intricate biological structures.