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Updated: Dec 18, 2025
Quantitative Measurement of Intrathecally Synthesized Proteins in Mice
Published on: November 29, 2019
Diagnostic accuracy of intrathecal kappa free light chains compared with OCBs in MS
Frida Duell1, Björn Evertsson2, Faiez Al Nimer2
1From the Department of Neurobiology, Care Sciences and Society (F.D.), Department of Clinical Neuroscience (B.E., F.A.N., T.O., M.K., M.A.H., F.P.), and Department of Laboratory Medicine (M.H.), Karolinska Institutet; Department of Clinical Chemistry (F.D., A.S., M.H.) and Department of Neurology (B.E., T.O., M.K., M.A.H., F.P.), Karolinska University Hospital; and Unit of Medical Statistics (D.O.), Department of Learning, Informatics, Management and Ethics (LIME), Karolinska Institutet, Stockholm, Sweden. frida.duell@sll.se.
Objective:
To determine what kappa free light chain (KFLC) metric has the highest capacity to separate healthy patients from patients with MS, we evaluated the sensitivity, specificity, and the overall diagnostic accuracy of 4 different KFLC metrics. To assess the usefulness of KFLC in the diagnostics of MS, we compared the different KFLC metrics with oligoclonal bands (OCBs), the current gold standard biochemical method to demonstrate intrathecal antibody production.
Methods:
CSF and plasma were collected from patients with confirmed or suspected MS, other neurological diseases, as well as symptomatic and healthy controls between May 2017 and May 2018 (n = 335) at the Department of Neurology, Karolinska University Hospital, as part of routine diagnostic workup. KFLC analysis and isoelectric focusing for the detection of oligoclonal bands (OCB) were determined and correlated with diagnosis. Receiver operating characteristic (ROC) curve analysis was used to determine accuracy.
Results:
OCBs yielded a sensitivity of 87% and a specificity of 100%. All KFLC metrics showed a high sensitivity (89%-95%) and specificity (95%-100%). Using the optimal cutoff according to the Youden Index resulted for the KFLC intrathecal fraction in a cutoff of -0.41 with a sensitivity of 95% and a specificity of 97% and for CSF KFLC/CSF albumin with a cutoff of 1.93 × 10-3 with a sensitivity of 94% and specificity of 100%.
Conclusion:
All evaluated KFLC metrics have excellent accuracy, and both KFLC intrathecal fraction and CSF KFLC/CSF albumin are at least as good as OCB in separating patients with MS from a control group.
Classification Of Evidence:
This study provides Class III evidence that CSF KFLC accurately distinguishes patients with MS from healthy controls.
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