Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hardware-Attentive Programmable Fourier Ptychography Enables Task-Adaptive Label-Free Virtual Staining.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Correction to "Thiolate DNAzymes on Gold Nanoparticles for Isothermal Amplification and Detection of Mesothelioma-derived Exosomal PD-L1 mRNA".

Analytical chemistry·2026

Same author

Binocular vision fusion enhanced 3D NIR-II <i>in vivo</i> imaging of bone and vessel networks.

Fundamental research·2026

Same author

Predicting the future of urban ecological resilience in China's Yellow River Basin: a machine learning approach.

Scientific reports·2026

Same author

Spatial transcriptomics identifies fibroblast-T cell crosstalk as a driver of Th2 polarization in allergic rhinitis.

Frontiers in immunology·2026

Same author

Beads-on-a-Tip testing for ultrasensitive antigen detection across a large dynamic range.

Smart molecules : open access·2026

Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026

Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026

Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026

Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026

Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026

Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 18, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Emerging technologies for profiling extracellular vesicle heterogeneity.

Guan Huang¹, Gungun Lin, Ying Zhu

¹Institute for Biomedical Materials and Devices, Faculty of Science, The University of Technology Sydney, Ultimo, New South Wales 2007, Australia. gungun.lin@uts.edu.au.

|June 16, 2020

Summary

This summary is machine-generated.

Extracellular vesicles (EVs) carry vital information but vary greatly. This review explores methods to profile EV heterogeneity, crucial for understanding their biological roles and applications.

More Related Videos

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Dec 18, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

Area of Science:

Biochemistry
Cell Biology
Molecular Biology

Background:

Extracellular vesicles (EVs) are secreted by cells and found in bodily fluids.
EVs contain diverse biomolecules like proteins, lipids, and nucleic acids.
EV populations exhibit significant heterogeneity in biogenesis, characteristics, and cargo.

Purpose of the Study:

To review recent methods for isolating and profiling extracellular vesicle (EV) heterogeneity.
To identify challenges in studying EV heterogeneity.
To highlight the importance of single-cell EV profiling and imaging.

Main Methods:

Survey of recent literature on EV isolation and characterization techniques.
Analysis of methods addressing EV heterogeneity.
Discussion of single-cell and single-EV analysis approaches.

Main Results:

Existing methods for EV isolation and profiling face challenges in capturing heterogeneity.
Single-cell EV profiling and advanced imaging offer promising avenues for detailed analysis.
Understanding EV heterogeneity is key to unlocking their therapeutic and diagnostic potential.

Conclusions:

Advanced techniques are needed to accurately profile the heterogeneity of extracellular vesicles (EVs).
Single-EV analysis holds promise for resolving complex EV populations.
Further research into EV heterogeneity will advance their clinical applications.