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Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Updated: Dec 18, 2025

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

Single-molecule methods in structural DNA nanotechnology.

Casey M Platnich¹, Felix J Rizzuto¹, Gonzalo Cosa¹

¹Department of Chemistry, McGill University, 801 Sherbrooke St. W, Montreal, Quebec H3A 0B8, Canada. gonzalo.cosa@mcgill.ca hanadi.sleiman@mcgill.ca.

Chemical Society Reviews

|June 16, 2020

Summary

This summary is machine-generated.

Advanced single-molecule techniques offer precise visualization of DNA nanostructures. Combining static and dynamic methods reveals detailed properties, enabling new DNA-based materials for diverse applications.

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Area of Science:

Biotechnology
Nanotechnology
Molecular Biology

Background:

Single-molecule visualization has advanced significantly, enabling detailed analysis.
DNA nanotechnology utilizes programmable nucleic acid interactions to build complex structures.
Characterizing these nanostructures requires sophisticated analytical methods.

Purpose of the Study:

To review how static and dynamic single-molecule techniques complement each other.
To demonstrate their application in characterizing DNA nanoarchitectures.
To highlight the insights gained into chemical and structural behaviors.

Main Methods:

Electron microscopy
Fluorescence microscopy
Electrical spectroscopy
Force spectroscopy

Main Results:

Single-molecule techniques provide high-resolution data on DNA nanoconstructs.
Complementarity of methods offers a unified view of nanoarchitectures.
Analysis reveals distribution of properties, not just averages.
Bulk properties are shown to arise from collective individual behaviors.

Conclusions:

Integrated single-molecule approaches yield unprecedented insights into DNA nanoarchitectures.
This synergy drives innovation in DNA-based materials.
Applications include sensors, delivery vehicles, machinery, and scaffolds.