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Whole Vitreous Humor Dissection for Vitreodynamic Analysis
Published on: May 24, 2015
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A NOVEL VITREOUS CULTURE TECHNIQUE
Jennifer Khong1, Brian C Joondeph
1Colorado Retina Associates, PC, Denver, Colorado.
Retinal Cases & Brief Reports
|June 17, 2020
Summary
A new method using the ESwab system for collecting vitreous samples after a vitreous tap and injection significantly improves the chances of a positive culture in infectious endophthalmitis cases.
Area of Science:
- Ophthalmology
- Microbiology
Background:
- Infectious endophthalmitis requires timely and accurate diagnosis.
- Traditional methods of vitreous specimen transport can lead to delays and reduced culture yield.
Purpose of the Study:
- To describe a novel method for collecting and transporting vitreous specimens.
- To evaluate the efficacy of the ESwab system for this purpose.
Main Methods:
- A case series of four patients with presumed infectious endophthalmitis.
- Vitreous specimens were collected via in-office vitreous tap and antibiotic injection.
- Specimens were transferred into the ESwab liquid-based collection and transport system.
Main Results:
- Three out of four cases (75%) showed a positive culture.
- The ESwab system facilitated sample collection and transport.
- The method proved effective even with potential delays in laboratory processing.
Conclusions:
- The ESwab system is a simple and effective method for vitreous specimen collection.
- This technique increases the likelihood of positive cultures in infectious endophthalmitis.
- It is particularly beneficial when there are delays in laboratory analysis.
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