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Related Experiment Video

Updated: Dec 17, 2025

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

Published on: October 7, 2025

A platform for multiplexed colorimetric microRNA detection using shape-encoded hydrogel particles.

Nidhi Juthani¹, Patrick S Doyle

¹Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. pdoyle@mit.edu.

|June 23, 2020

Summary

This summary is machine-generated.

This study introduces a novel hydrogel microparticle platform for sensitive and multiplexed microRNA detection. The system uses a reporter enzyme and can be imaged with a phone camera, showing promise for clinical diagnostics.

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Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

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Last Updated: Dec 17, 2025

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

Published on: October 7, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Published on: August 3, 2011

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

Area of Science:

Biotechnology
Analytical Chemistry
Molecular Diagnostics

Background:

Developing sensitive and multiplexed detection methods for biomarkers like microRNA (miRNA) is crucial for early disease diagnosis.
Existing methods often require complex instrumentation and extensive sample preparation.

Purpose of the Study:

To develop a novel platform for sensitive, multiplexed detection of miRNA using shape-encoded hydrogel microparticles.
To demonstrate the utility of this platform for analyzing clinical samples.

Main Methods:

Utilized a reporter enzyme system producing a chromogenic indigo precipitate within hydrogel microparticles.
Employed shape-encoding of hydrogel microparticles for multiplexed target detection.
Developed a detection method compatible with simple phone camera imaging.

Main Results:

Achieved sensitive detection of miRNA down to 0.22 fmol.
Demonstrated successful multiplexed miRNA detection from total RNA extracted from colon cancer and normal adjacent tissues.
Confirmed that the hydrogel network preserves rapid target binding kinetics.

Conclusions:

The developed hydrogel microparticle platform offers a sensitive, multiplexed, and user-friendly approach for miRNA detection.
This technology shows potential for point-of-care diagnostics and analysis of clinical samples.
The system's compatibility with phone cameras enhances its accessibility for broader applications.