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Cell-Laden Hydrogels for Multikingdom 3D Printing.

Trevor G Johnston1, Jacob P Fillman1, Hans Priks2

  • 1Department of Chemistry, University of Washington, Box 351700, Seattle, WA, 98195-1700, USA.

Macromolecular Bioscience
|June 23, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed a new 3D-printable hydrogel for creating advanced living materials. This innovation enables precise spatial arrangement of multiple microorganisms, paving the way for novel bioreactors and biosensors.

Keywords:
3D printingconsortiahydrogelmicrobesspatial organization

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Area of Science:

  • Biomaterials Engineering
  • Synthetic Biology
  • Microbiology

Background:

  • Living materials integrate live cells into matrices for applications like chemical production.
  • Current challenges include poor structural integrity, patterning difficulties, and limited control over microbial consortia composition and arrangement.
  • There is a need for robust, patternable living material systems capable of housing diverse microbial communities.

Purpose of the Study:

  • To characterize a Pluronic F127-based hydrogel system for encapsulating diverse microorganisms (algae, yeast, bacteria).
  • To demonstrate the hydrogel's suitability for additive manufacturing, specifically direct write 3D-printing, for precise cell patterning.
  • To explore the behavior of immobilized cells within this novel living material matrix.

Main Methods:

  • Encapsulation of algae, yeast, and bacteria within a Pluronic F127 hydrogel.
  • Characterization of the hydrogel's properties for cell viability and matrix housing.
  • Utilizing direct write 3D-printing technology to spatially arrange encapsulated cells within the hydrogel.
  • Investigating cell behavior and interactions within the 3D-printed living material constructs.

Main Results:

  • The Pluronic F127 hydrogel effectively houses and sustains the viability of encapsulated algae, yeast, and bacteria.
  • The hydrogel system is compatible with direct write 3D-printing, allowing for precise spatial arrangement of multiple microbial types.
  • Complex, immobilized microbial consortia with controlled spatial organization were successfully created.
  • Enhanced understanding of cellular behavior within engineered living material matrices was achieved.

Conclusions:

  • The developed Pluronic F127 hydrogel system offers a robust and patternable platform for creating advanced living materials.
  • 3D-printing enables unprecedented control over the spatial organization of multikingdom microbial consortia.
  • These living materials hold significant potential for applications in immobilized bioreactors and biosensing technologies.