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Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy
Published on: April 21, 2023
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A Metabolomics Study of Hypoxia Ischemia during Mouse Brain Development Using Hyperpolarized 13C
Alkisti Mikrogeorgiou1, Yiran Chen2,3, Byong Sop Lee4
1Department of Neurology, University of California, San Francisco, California, USA.
Developmental Neuroscience
|June 23, 2020
Summary
Hyperpolarized 13C magnetic resonance spectroscopy (MRS) revealed reduced pyruvate delivery and increased lactate in infant mouse brains after hypoxia-ischemia (HI). These metabolic changes resolved as the brain matured, suggesting MRS as a potential biomarker for HI injury.
Area of Science:
- Neuroscience
- Biochemistry
- Medical Imaging
Background:
- Hyperpolarized 13C magnetic resonance spectroscopy (MRS) offers real-time insights into brain metabolism.
- Hypoxia-ischemia (HI) is a critical condition affecting brain development.
Purpose of the Study:
- To investigate real-time metabolic changes in the brain following hypoxia-ischemia (HI) using hyperpolarized 13C MRS.
- To monitor the evolution of HI injury during brain maturation in a mouse model.
Main Methods:
- Unilateral HI was induced in mice on postnatal day 10.
- Hyperpolarized 13C-1-labeled pyruvate was administered intravenously.
- Chemical-shift imaging was used to acquire spectral-spatial data, comparing injured and contralateral hemispheres at P10, P17, and P31.
Main Results:
- A significant reduction in pyruvate delivery and an elevated lactate-to-pyruvate ratio were observed in the HI hemisphere at P10.
- These metabolic differences diminished by P17 and resolved by P31.
- Normalized lactate levels increased from P10 to P31 in both HI and sham mice.
Conclusions:
- Hyperpolarized 13C MRS can detect and monitor the progression of HI injury during brain development.
- This metabolic imaging technique shows promise as a sensitive biomarker for brain injury.
