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Updated: Dec 17, 2025

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
Published on: February 5, 2018
Enzymatic modular synthesis and microarray assay of poly-N-acetyllactosamine derivatives
Congcong Chen1, Shuaishuai Wang, Madhusudhan Reddy Gadi
1School of Pharmaceutical Sciences, Key Laboratory of Chemical Biology (Ministry of Education), Cheeloo College of Medicine, Shandong University, Jinan 250012, China. fswang@sdu.edu.cn.
Abstract:
A facile enzymatic modular assembly strategy for the preparative-scale synthesis of poly-N-acetyllactosamine (poly-LacNAc) glycans with varied lengths and designed sialylation and/or fucosylation patterns is described. These glycans were printed as a microarray to investigate their interactions with a panel of glycan binding proteins (GBPs). Binding affinities revealed that the avidity of GBPs could be largely affected by the length and the patterns of sialylation and fucosylation.

