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Exploring nanofibrous self-assembling peptide hydrogels using mouse myoblast cells for three-dimensional bioprinting
Wafaa Arab1, Kowther Kahin1,2, Zainab Khan1,2
1Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
International Journal of Bioprinting
|June 30, 2020
Summary
Three-dimensional bioprinting using peptide hydrogels shows promise for skeletal muscle repair. This study demonstrates their potential as scaffolds for regenerating muscle tissue after volumetric muscle loss.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Volumetric muscle loss (VML) impairs skeletal muscle self-healing.
- Traditional regenerative therapies face limitations.
- Three-dimensional (3D) bioprinting offers a novel approach for tissue transplantation and muscle repair.
Purpose of the Study:
- To evaluate ultrashort self-assembling peptide hydrogels as biomaterials for muscle tissue formation.
- To assess cell viability, proliferation, and differentiation within 3D peptide hydrogel scaffolds.
- To investigate the potential of 3D bioprinted constructs for skeletal muscle regeneration.
Main Methods:
- Two ultrashort peptide sequences were analyzed with C2C12 muscle myoblast cells in 3D culture.
- Cell viability, proliferation, and differentiation were assessed.
- Peptide hydrogels were extruded via robotic 3D bioprinting to create cell-laden constructs.
- Live/dead assays were performed on the 3D bioprinted structures.
Main Results:
- The peptide hydrogels supported muscle myoblast cell viability and proliferation in 3D culture.
- 3D bioprinted constructs demonstrated good cell viability.
- The peptide hydrogel scaffolds facilitated myotube formation, a key step in muscle repair.
Conclusions:
- Ultrashort self-assembling peptide hydrogels are biocompatible and suitable for 3D cell culture.
- Robotic 3D bioprinting of these peptides can create functional tissue platforms.
- These findings highlight the potential of 3D bioprinted peptide hydrogels for skeletal muscle tissue regeneration.
