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Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation
Enkhtuul Gantumur1, Masaki Nakahata1, Masaru Kojima1
1Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan.
International Journal of Bioprinting
|June 30, 2020
Summary
This study introduces a novel bioprinting method using enzyme-catalyzed cross-linking for stable 3D hydrogel constructs. The bioprinted materials support cell viability and can be modified for cell adhesion, demonstrating potential for tissue engineering.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Bioprinting Technology
Background:
- Developing stable 3D hydrogel constructs for bioprinting is challenging.
- Existing methods often struggle with maintaining structural integrity and cell viability.
- A need exists for efficient cross-linking strategies in bioink formulations.
Purpose of the Study:
- To present an extrusion-based bioprinting approach.
- To achieve bioink stabilization via horseradish peroxidase (HRP)-catalyzed cross-linking.
- To demonstrate the fabrication of stable, cell-laden 3D hydrogel constructs.
Main Methods:
- Utilized bioinks containing living cells, HRP, glucose, phenolic hydroxyl (Ph)-modified alginate, and cellulose nanofiber.
- Employed extrusion-based bioprinting to fabricate 3D lattice and human nose-shaped constructs.
- Assessed construct stability in cell culture medium and cell viability over 7 days.
Main Results:
- Successfully fabricated stable 3D hydrogel constructs with good structural integrity for over a week.
- Demonstrated sustained viability of encapsulated mouse fibroblasts after 7 days of culture.
- Showcased the ability to modify construct surfaces for enhanced cell adhesion through secondary cross-linking.
Conclusions:
- The HRP-catalyzed cross-linking method provides effective stabilization for 3D bioprinted constructs.
- The developed approach supports long-term cell viability within the hydrogel.
- This bioprinting strategy offers a versatile platform for tissue engineering applications.
