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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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MALDI-TOF Mass Spectrometry01:19

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Related Experiment Video

Updated: Dec 17, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

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Single-cell protein analysis by mass spectrometry.

Nikolai Slavov1

  • 1Department of Bioengineering, Northeastern University, Boston, MA, 02115, USA; Barnett Institute, Northeastern University, Boston, MA, 02115, USA; Department of Biology, Northeastern University, Boston, MA, 02115, USA.

Current Opinion in Chemical Biology
|June 30, 2020
PubMed
Summary
This summary is machine-generated.

Single-cell protein analysis is now feasible using mass spectrometry, overcoming previous limitations in sensitivity and throughput. This advancement allows for the quantification of thousands of proteins across numerous single cells, paving the way for deeper biological insights.

Keywords:
Isobaric carrierMass-spectrometrySample preparationSingle-cell analysisSingle-cell proteomicsSystems biology

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Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
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Area of Science:

  • Proteomics
  • Cellular Biology
  • Biotechnology

Background:

  • Single-cell analysis is crucial for understanding human physiology and pathology.
  • Existing analytical methods for single cells, especially for proteins, suffer from low sensitivity and throughput.
  • Mass spectrometry offers powerful protein analysis but faces challenges in single-cell applications.

Purpose of the Study:

  • To overcome the limitations of single-cell protein analysis.
  • To enable high-throughput quantification of proteins in thousands of single cells.
  • To establish a foundation for advanced single-cell proteomic studies.

Main Methods:

  • Development of Single-Cell ProtEomics (SCoPE) design multiplexing.
  • Implementation of clean, automated, and miniaturized sample preparation techniques.
  • Application of mass spectrometry for protein and peptide detection and sequencing.

Main Results:

  • Successful quantification of thousands of proteins across many single cells.
  • Demonstration of solutions addressing challenges in single-cell mass spectrometry.
  • Establishment of a scalable platform for single-cell proteomic analysis.

Conclusions:

  • The SCoPE concept significantly advances single-cell protein analysis capabilities.
  • Future applications include analyzing subcellular organelles and posttranslational modifications.
  • Increased multiplexing will enhance throughput and reduce costs for single-cell proteomics.