Target-Driven Nanozyme Growth in TiO₂ Nanochannels for Improving Selectivity in Electrochemical Biosensing

Nanozymes have been used in colorimetric and electrochemical sensing because of their low cost and high stability. However, the wide applications of nanozymes in sensing devices are largely limited due to their poor selectivity. In this study, unlike traditional methods using prepared nanozymes for target detection, we designed a target-driven nanozyme growth strategy in TiO₂ nanochannels to detect analytes. Using telomerase as an example, the established recognition event was used to expand the photocatalytic activity of TiO₂ to visible-light region, thus triggering Prussian blue nanoparticle (PBNP) growth in visible light. Benefiting from the peroxidase (POD)-like activity of PBNPs, the uncharged 3,5,3',5'-tetramethylbenzidine (TMB) is oxidized to positively charged oxTMB, which induces significant ionic transport changes in nanochannels, and thus in turn provides information about telomerase activity. Such a nanozyme-triggered sensing system exhibited excellent performance in telomerase detection in urine specimens from patients with bladder cancer. This innovative target-driven signal generation strategy might provide a new method for applying nanozymes in developing sensitive, rapid, and accurate biological sensing systems.