[Realization Process of Nitritation and Changes in Sludge Characteristics in Granular Sludge Reactor for Low Strength...

The realization process of nitritation was studied in a CSTR reactor seeding with nitrification granular sludge to treat low ammonia sewage. During the operation period, the physical and chemical properties, the spatial distribution of functional microbes, and the activity of the granular sludge were also investigated to elaborate the main factors for the stability of nitritation. The results showed that nitritation can be successfully achieved and maintained by the cooperative controlling of nitrogen loading rate (NLR) and dissolved oxygen (DO) levels, and the nitrite accumulation rate was over 80%. The obtained nitritation granular sludge was brownish yellow, showing a smooth, full ellipsoid or sphere, and the microorganisms on the surface of the particles were mainly cocci; the average particle size was 1.3 mm, and the average sedimentation rate was 71.3 m·h^-1. Batch tests showed that there was a significant stratified distribution structure in granular sludge (particle size >0.8 mm), the ammonia-oxidizing bacteria (AOB) mainly occupied the surface space of the particles, and the nitrite-oxidizing bacteria (NOB) were mainly distributed inside the particles. Flocs or small-size sludge (particle size<0.8 mm) and granular sludge (particle size >0.8 mm) exhibit different spatial distribution characteristics of microorganisms. In the granular sludge reactor, well stratification of the nitrifier guilds, high level of residual ammonia concentrations in effluent (15-33 mg·L^-1), or low ratio between DO and NH₄⁺-N concentrations (0.08-0.15) should be key influencing factors in the process of achieving nitritation.