An open-hardware sample mounting solution for inverted light-sheet microscopes with large detection objective lenses

Implementations of light-sheet microscopes are often incompatible with standard methods of sample mounting. Light-sheet microscopy uses orthogonal illumination and detection to create a thin sheet of light which does not illuminate the sample outside of the depth of field of the detection axis. Typically, this configuration involves a pair of orthogonal objectives which constrains the positioning and length of cover slips in the range of the detection objective. Here, we present an open-hardware sample mounting system for light-sheet microscopes using large detection objectives, built using 3D printed components and demonstrate the chamber's efficacy on live biological samples in a custom light-sheet microscope. LAY DESCRIPTION: Implementations of light-sheet microscopes are often incompatible with standard methods of sample mounting. Light-sheet microscopy creates a thin sheet of light at a certain depth of field within a volumetric sample. Typically, this configuration involves a pair of orthogonal objectives which constrains the positioning of samples and sample-mounting apparatus in range of the detection objective. To overcome the limitations of this setup, we present an open-hardware sample mounting system for light-sheet microscopes using large detection objectives, built using 3D printed components and demonstrate the chamber's efficacy on live biological samples in a custom light-sheet microscope.