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Dynamic conjugate F-SHARP microscopy.

Ioannis N Papadopoulos1, Jean-Sebastien Jouhanneau2, Naoya Takahashi3

  • 1Charité - Universitätsmedizin Berlin, Einstein Center for Neurosciences, NeuroCure Cluster of Excellence, Charitéplatz 1, 10117 Berlin, Germany.

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|July 9, 2020
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Summary
This summary is machine-generated.

This study introduces a new optical microscopy technique, focus scanning holographic aberration probing (F-SHARP), to image deep into tissues. F-SHARP overcomes scattering and aberrations, enabling high-resolution imaging over large fields of view in biological samples.

Keywords:
Interference microscopyMultiphoton microscopy

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Area of Science:

  • Biomedical Optics
  • Microscopy Techniques
  • Neuroimaging

Background:

  • Optical microscopy is crucial in biomedical research but struggles with deep tissue penetration due to scattering and aberrations.
  • Existing wavefront-shaping methods improve imaging but are limited by small fields of view (FOVs) and fixed correction depths.
  • Conjugate wavefront shaping offers potential for wider FOVs but lacks dynamic adaptability for varying depths.

Purpose of the Study:

  • To develop an advanced conjugate wavefront-shaping technique for enhanced deep-tissue optical microscopy.
  • To enable high-resolution imaging over extended fields of view (FOVs) beyond current limitations.
  • To create a versatile and adaptable system compatible with various microscopy setups.

Main Methods:

  • Implementation of a novel conjugate wavefront-shaping scheme named focus scanning holographic aberration probing (F-SHARP).
  • Integration of F-SHARP into a compact system adaptable to commercial and custom two-photon microscopes.
  • Demonstration of the technique's efficacy in imaging through scattering biological tissues.

Main Results:

  • Achieved high-resolution imaging over extended fields of view (>80 µm).
  • Successfully imaged deeper than 400 µm within a mouse brain through a thinned skull.
  • Demonstrated the method's capability to overcome aberrations and scattering in deep tissues.

Conclusions:

  • F-SHARP significantly enhances the depth and field of view for optical microscopy in scattering media.
  • The developed compact system offers a practical solution for advanced deep-tissue imaging in biomedical applications.
  • This technique holds promise for future in vivo neuroimaging and other deep-tissue investigations.