Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wildfire ash impacts the early development of the sea urchin Paracentrotus lividus.

Marine environmental research·2026
Same author

Rosy Discolouration in an Alpine Chapel: Beyond Salt Dependence.

Microbial ecology·2026
Same author

Cord-blood black carbon burden is associated with coordinated inflammatory and heme-metabolic transcriptional programs at birth in Bradford, United Kingdom.

The Science of the total environment·2026
Same author

Comparative real-world progression free survival of CDK4/6 inhibitors in HR+/HER2- breast cancer patients with bone metastases.

The oncologist·2026
Same author

Multidisciplinary Clinic Approach Improves Immunotherapy Treatment Outcomes in Unresectable Hepatocellular Carcinoma: A Multicentre Retrospective Study.

Liver cancer·2026
Same author

Advancing Italian biophysics: insights from the 27th SIBPA congress.

European biophysics journal : EBJ·2026
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Dec 15, 2025

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.8K

Two-photon image-scanning microscopy with SPAD array and blind image reconstruction.

Sami V Koho1,2,3, Eli Slenders1,4,3, Giorgio Tortarolo1,5

  • 1Molecular Microscopy and Spectroscopy, Istituto Italiano di Tecnologia, Genoa, Italy.

Biomedical Optics Express
|July 9, 2020
PubMed
Summary
This summary is machine-generated.

We developed a new two-photon excitation image scanning microscopy (2PE-ISM) technique. This method significantly enhances resolution and image quality in thick biological samples without user calibration.

More Related Videos

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.4K
Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

11.8K

Related Experiment Videos

Last Updated: Dec 15, 2025

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.8K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.4K
Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

11.8K

Area of Science:

  • Biomedical Imaging
  • Optical Microscopy
  • Super-resolution Techniques

Background:

  • Two-photon excitation (2PE) microscopy is crucial for imaging thick biological tissues.
  • Standard 2PE microscopy suffers from limited spatial resolution compared to confocal microscopy.
  • Improving resolution and image quality in 2PE microscopy is essential for detailed biological studies.

Purpose of the Study:

  • To introduce a straightforward implementation of 2PE image scanning microscopy (2PE-ISM).
  • To enhance the effective spatial resolution and overall image quality of 2PE microscopy.
  • To provide a user-friendly 2PE-ISM technique requiring no calibration.

Main Methods:

  • Utilizing a single-photon avalanche diode (SPAD) array detector.
  • Implementing a novel blind image reconstruction method with adaptive pixel reassignment.
  • Integrating Fourier ring correlation (FRC) based semi-blind deconvolution and automatic background correction.

Main Results:

  • Achieved an effective resolution increase of approximately 1.6 times deep within thick samples using adaptive pixel reassignment.
  • Further enhanced effective resolution by a factor of approximately 2 using FRC-based deconvolution.
  • Automatic background correction significantly improved image quality, particularly in noisy datasets.

Conclusions:

  • The proposed 2PE-ISM offers a significant advancement in optical microscopy for thick biological samples.
  • The technique provides enhanced resolution and image quality without requiring user calibration, improving practical usability.
  • This method represents a valuable tool for researchers needing high-resolution imaging in challenging biological specimens.