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Simple and label-free strategy for terminal transferase assay using a personal glucose meter
Hyo Yong Kim1, Jayeon Song, Ki Soo Park
1Department of Chemical and Biomolecular Engineering (BK21+ Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. hgpark@kaist.ac.kr.
Chemical Communications (Cambridge, England)
|July 9, 2020
Abstract:
We herein developed a simple personal glucose meter (PGM)-based method for terminal transferase (TdT) activity assay by utilizing the glucose oxidase (GOx)-mimicking activity of cerium oxide nanoparticles (CeO2 NPs). Using this strategy, the TdT activity was reliably determined down to 0.7 U mL-1 with high selectivity against other non-specific enzymes.
