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Related Experiment Video

Updated: Dec 15, 2025

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

Published on: October 4, 2017

Gel Microrods for 3D Tissue Printing.

Shaohua Ma¹, Nobina Mukherjee¹, Ellina Mikhailova¹

¹Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.

Advanced Biosystems

|July 11, 2020

Summary

This summary is machine-generated.

Researchers developed a microfluidic method to create ordered 3D gel structures from microrods. This breakthrough enables the engineering of complex, cell-laden replica tissues with precise architectures for advanced biomedical applications.

Keywords:

3D-printing Matrigel gelatin methacrylate microrods

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Last Updated: Dec 15, 2025

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

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Published on: October 4, 2017

Interlinked Macroporous 3D Scaffolds from Microgel Rods

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Published on: June 16, 2022

Micropatterning and Assembly of 3D Microvessels

Micropatterning and Assembly of 3D Microvessels

Published on: September 9, 2016

Area of Science:

Biomaterials Engineering
Tissue Engineering
Microfluidics

Background:

3D ordering of anisotropic gel objects is crucial for engineering replica tissues.
Current methods lack the ability to achieve large-scale 3D ordering of gel microrods.
Soft and rigid gel microrods are needed for diverse tissue engineering applications.

Purpose of the Study:

To develop a method for large-scale 3D ordering of anisotropic gel microrods.
To engineer robust 3D structures with organized gel microrods for tissue mimicry.
To assess the viability and behavior of cells encapsulated within the gel microrods.

Main Methods:

Monodisperse gel microrods (gelatin methacrylate or Matrigel) were fabricated using a droplet-based microfluidics tubing system.
Microrods of varying dimensions (50-300 µm width, 1-3 mm length) were produced at high throughput.
The microrods were printed into centimeter-scale 3D structures with patterned arrangements.

Main Results:

Fabrication of homogeneous, Janus, and ternary gel microrods was achieved.
Produced microrods were successfully printed into centimeter-scale 3D structures with organized patterns.
Encapsulated mammalian cells remained viable, proliferated, and migrated within the microrods.

Conclusions:

The microfluidic method enables scalable 3D ordering of gel microrods for tissue engineering.
This technique allows for the creation of complex, nature-mimicking tissue architectures.
Gel microrods offer a versatile platform for developing advanced printed tissues.