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Antibody microarray analysis of serum inflammatory cytokines in patients with calcific aortic valve disease

Bo Fu1, Yuhui Zhang1,2, Qingliang Chen1

  • 1Department of Cardiovascular Surgery, Tianjin Chest Hospital, Tianjin, China.

Annals of Translational Medicine
|July 11, 2020
PubMed

Insights

Calcific aortic valve disease (CAVD) involves inflammation. Researchers identified four key inflammatory markers, B-Lymphocyte Chemoattractant (BLC), Interleukin (IL)-12p40, monokine inducible by γ interferon (MIG), and Macrophage inflammatory protein (MIP)-1delta, as potential indicators for CAVD assessment.

Area of Science:

  • Cardiovascular Research
  • Immunology
  • Biomarker Discovery

Background:

  • Calcific aortic valve disease (CAVD) is a prevalent condition in the elderly, leading to aortic valve stenosis and significant mortality.
  • CAVD pathogenesis involves inflammation, apoptosis, lipid deposition, and matrix remodeling.
  • Current understanding necessitates novel diagnostic markers for CAVD.

Purpose of the Study:

  • To investigate and analyze the expression of serum inflammatory factors in patients with CAVD.
  • To identify potential serum biomarkers for the early detection and assessment of CAVD.

Main Methods:

  • A cohort of 258 patients was categorized into control, coronary artery disease (CAD), and CAVD groups.
  • Serum adipokine, cytokine, and chemokine profiles were analyzed using antibody microarray techniques.
  • Validation of key markers was performed using Real-time PCR, Western blot, and flow cytometry.

Main Results:

  • Significantly elevated levels of B-Lymphocyte Chemoattractant (BLC), Interleukin (IL)-12p40, monokine inducible by γ interferon (MIG), and Macrophage inflammatory protein (MIP)-1delta were observed in the CAVD group compared to control and CAD groups.
  • These four inflammatory markers were confirmed to originate from peripheral blood mononuclear cells.
  • The identified markers showed differential expression patterns relevant to CAVD.

Conclusions:

  • BLC, IL-12p40, MIG, and MIP-1delta demonstrate potential as reliable serum biomarkers for CAVD.
  • These findings may offer significant clinical implications for CAVD diagnosis and management.
  • Further research can explore the therapeutic targeting of these inflammatory pathways.
Abstract

Keywords:
Calcific aortic valve disease (CAVD)antibody microarraycoronary artery disease (CAD)

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