Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Radiomics-based optimization of target selection in CT-guided percutaneous lung cancer biopsy: a retrospective study.

Frontiers in oncology·2026
Same author

Evaluation of the contemporary cultural landscape based on multi-dimensional value coupling: Case study on the Grand Canal, Hangzhou section.

PloS one·2025
Same author

Understanding the role of urban block morphology in innovation vitality through explainable machine learning.

Scientific reports·2025
Same author

CRISPR-based Shuttle Cloning: A High-throughput Cloning Method.

Journal of visualized experiments : JoVE·2025
Same author

CRISPR-based shuttle cloning of 1397 human genes into UAS vectors.

Acta biochimica et biophysica Sinica·2025
Same author

Neuronal Intranuclear Inclusion Disease: A Confirmed Case Report and Analysis of MRI Characteristics in Three Typical Cases

Current medical imaging·2025

Related Experiment Video

Updated: Dec 15, 2025

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
08:27

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Published on: July 24, 2014

12.8K

Human-Induced Pluripotent Stem Cell Culture Methods Under cGMP Conditions.

Teresa Rivera1, Yuanyuan Zhao1, Yuhui Ni1

  • 1Allele Biotechnology and Pharmaceuticals, Inc., San Diego, California.

Current Protocols in Stem Cell Biology
|July 11, 2020
PubMed
Summary

Induced pluripotent stem cells (iPSCs) offer revolutionary cell therapy potential. This study details cGMP-compliant methods for culturing, examining, and storing these patient-specific cells, ensuring quality for regenerative medicine applications.

Keywords:
cGMPiPSCinduced pluripotent stem cellsmRNA reprogrammingxeno-free

More Related Videos

Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility
06:11

Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility

Published on: January 26, 2024

1.9K
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

8.2K

Related Experiment Videos

Last Updated: Dec 15, 2025

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
08:27

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Published on: July 24, 2014

12.8K
Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility
06:11

Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility

Published on: January 26, 2024

1.9K
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

8.2K

Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Biotechnology

Background:

  • Induced pluripotent stem cells (iPSCs) are crucial for patient-specific cell therapies.
  • Manufacturing iPSCs under current good manufacturing practice (cGMP) is essential for clinical translation.
  • Existing protocols require optimization for robust and consistent iPSC production.

Purpose of the Study:

  • To detail the cGMP-compliant methods for culturing, examining, and storing induced pluripotent stem cells.
  • To present a footprint-free, feeder-free, and xeno-free reprogramming and manufacturing platform.
  • To ensure the quality and consistency of iPSC lines for regenerative medicine.

Main Methods:

  • Utilizing a proprietary platform for footprint-free, feeder-free, and xeno-free iPSC generation under cGMP conditions.
  • Implementing fully cGMP-compliant procedures for tissue collection, reprogramming, expansion, banking, and quality control.
  • Employing cGMP-compliant reagents, materials, and equipment for iPSC culture, examination, and storage.

Main Results:

  • Established a robust platform for generating cGMP-compliant iPSC lines.
  • Detailed protocols for iPSC dissociation, culture, cryopreservation, and thawing.
  • Ensured the entire manufacturing process adheres to cGMP standards.

Conclusions:

  • The described methods facilitate the reliable production and maintenance of high-quality cGMP-iPSCs.
  • These protocols are critical for advancing cell therapy and regenerative medicine applications.
  • Allele Biotechnology's platform provides a powerful resource for patient-specific stem cell manufacturing.