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Improving laccase thermostability with aqueous natural deep eutectic solvents.

Astrid E Delorme1, Jean-Michel Andanson1, Vincent Verney1

  • 1Université Clermont Auvergne, CNRS, SIGMA Clermont, ICCF, F-63000 Clermont-Ferrand, France.

International Journal of Biological Macromolecules
|July 11, 2020
PubMed
Summary

Natural Deep Eutectic Solvents (NADESs) significantly enhance laccase enzyme stability. Incubating laccase in NADES media at high temperatures dramatically increases its residual activity, offering a green method for enzyme stabilization.

Keywords:
Deep eutectic solventsEnzyme stabilityLaccase

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Area of Science:

  • Biocatalysis
  • Enzyme Engineering
  • Green Chemistry

Background:

  • Industrial application of laccases is limited by enzyme inactivation under non-optimal conditions (neutral pH, 30-40°C).
  • Natural Deep Eutectic Solvents (NADESs) are sustainable reaction media with demonstrated enzyme compatibility.

Purpose of the Study:

  • To investigate the use of aqueous NADESs as incubation media to enhance laccase activity and thermal stability.
  • To evaluate the protective effect of NADESs against thermal inactivation of laccase from *T. versicolor*.

Main Methods:

  • Incubation of *T. versicolor* laccase in an aqueous betaine-xylitol NADES at 70°C for 15 minutes.
  • Comparison of residual enzyme activity after incubation with NADES versus individual components (betaine, xylitol) or no additive.

Main Results:

  • Pre-incubation in a 25 wt% betaine-xylitol NADES resulted in a nearly 10-fold increase in residual laccase activity compared to incubation without NADES.
  • The NADES demonstrated superior enzyme stabilization compared to its individual components, betaine and xylitol.
  • Significant enhancement of enzyme thermostability was observed.

Conclusions:

  • Aqueous NADESs provide a facile, cost-effective, and environmentally friendly method to significantly boost laccase thermostability.
  • NADESs offer a promising strategy to overcome operational limitations of laccases in industrial biocatalysis.