Method for Stimulation of Malignant Growth by Chronic Pain in Rat Lungs

We developed a method of reproducing malignant process in the lungs of rats with stimulation by chronic neurogenic pain. In white outbred male rats, chronic neurogenic pain was modeled by bilateral ligation of the sciatic nerves and in 45 days, a suspension of M1 sarcoma cells (10⁶ cells/liter in 0.3 ml physiological saline) was injected into the subclavian vein. In almost all rats receiving transplantation of tumor cells against the background of chronic neurogenic pain, tumor foci in the lungs were detected in 1.5-2.0 months and led to the death of the animals.