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Targeting Delivery System for Lactobacillus Plantarum Based on Functionalized Electrospun Nanofibers
Hongliang Yu1, Weihua Liu1, Dongmei Li1
1Department of Applied Chemistry, Northeast Agricultural University, Harbin 150000, China.
Polymers
|July 19, 2020
Summary
This study developed polylactic acid (PLA) nanofibers to protect probiotics. Encapsulating lactic acid bacteria in these nanofibers significantly improved their survival rate during simulated digestion.
Area of Science:
- Biomaterials Engineering
- Microbiology
- Food Science
Background:
- Probiotics offer health benefits, but their viability is often compromised by harsh environments.
- Developing effective encapsulation methods is crucial for enhancing probiotic survival and efficacy.
- Lactic acid bacteria, a common probiotic, require protection against adverse conditions.
Purpose of the Study:
- To improve the viability of lactic acid bacteria (probiotics) using novel encapsulation techniques.
- To explore the use of polylactic acid (PLA) nanofibers for probiotic delivery.
- To assess the effectiveness of coaxial electrospinning for encapsulating probiotics.
Main Methods:
- Coaxial electrospinning was employed to create polylactic acid (PLA) nanofibers.
- Lactic acid bacteria were encapsulated within PLA nanofibers using fructooligosaccharides (FOS) as shell materials.
- Characterization involved scanning electron microscopy (SEM), transmission electron microscopy (TEM), laser scanning confocal microscopy, and water contact angle tests.
- In vitro simulated digestion tests were conducted to evaluate probiotic survival rates.
Main Results:
- Successful encapsulation of lactic acid bacteria within coaxial electrospun PLA nanofibers was confirmed by microscopy.
- The resulting nanofiber films demonstrated good hydrophobicity, indicated by water contact angle measurements.
- An in vitro simulated digestion test showed a survival rate exceeding 72% for the encapsulated lactic acid bacteria.
- The study demonstrated improved probiotic viability through this encapsulation method.
Conclusions:
- Coaxial electrospun PLA nanofibers provide an effective method for enhancing probiotic viability.
- This technique offers a novel approach for the encapsulation and delivery of sensitive bioactive substances.
- The developed method holds potential for improving the efficacy of probiotic supplements and functional foods.
