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Monoclonal Antibody Against ROR1 Induces Apoptosis in Human Bladder Carcinoma Cells
Ali-Ahmad Bayat1, Niloufar Sadeghi1, Ramina Fatemi1
1Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Background:
Receptor tyrosine kinase-like Orphan Receptor 1 (ROR1) is one of the promising cell surface antigens for targeting cancer cells. The aim of this study was to evaluate ROR1 cell surface expression in bladder cancer cells using a murine anti-ROR1 monoclonal antibody (mAb) called 5F1-B10 as well as investigate its potential in apoptosis induction.
Methods:
Expression of ROR1 in two human bladder cell lines, 5637 and EJ138, as well as a non-cancerous human cell line, Human Fetal Foreskin Fibroblast (HFFF), was examined by flow cytometry and immunocytochemistry. Immunohistochemical staining of cancer and normal bladder tissues was also performed.
Results:
The flow cytometry results showed that 5F1-B10 mAb could recognize ROR1 molecules in 86.1% and 45.6% of 5637 and EJ138 cells, respectively. The expression level of ROR1 was 5.49% in HFFF cells. The immunocytochemistry and immunohistochemistry staining results also confirmed the presence of ROR1 on the surface of both bladder cancer cells and tissues, respectively. The obtained data from apoptosis assay demonstrated that 5F1-B10 mAb could induce apoptosis in both 5637 and EJ138 cell lines.
Conclusion:
Taken together, our finding indicates the role of ROR1 in bladder cancer cell survival and suggests this receptor might be a promising target for developing novel therapeutic agents against bladder carcinoma.
Insights
Receptor tyrosine kinase-like Orphan Receptor 1 (ROR1) is expressed on bladder cancer cells. A monoclonal antibody targeting ROR1 induced apoptosis, suggesting ROR1 as a therapeutic target for bladder carcinoma.
Area of Science:
- Oncology
- Molecular Biology
- Immunology
Background:
- Receptor tyrosine kinase-like Orphan Receptor 1 (ROR1) is a cell surface antigen with potential for cancer cell targeting.
- ROR1 expression and its role in cancer survival are areas of active research.
Purpose of the Study:
- To evaluate ROR1 cell surface expression in bladder cancer.
- To investigate the potential of a specific anti-ROR1 monoclonal antibody (5F1-B10) in inducing apoptosis.
Main Methods:
- Flow cytometry and immunocytochemistry were used to assess ROR1 expression in bladder cancer cell lines (5637, EJ138) and a control cell line (HFFF).
- Immunohistochemical staining was performed on bladder tissues.
- Apoptosis assays were conducted to evaluate the effect of the 5F1-B10 mAb.
Main Results:
- The 5F1-B10 mAb recognized ROR1 on 86.1% of 5637 cells and 45.6% of EJ138 cells.
- ROR1 expression was also detected in non-cancerous HFFF cells at 5.49%.
- Immunohistochemistry confirmed ROR1 presence on bladder cancer cells and tissues. The 5F1-B10 mAb induced apoptosis in both tested bladder cancer cell lines.
Conclusions:
- ROR1 plays a role in bladder cancer cell survival.
- ROR1 represents a promising therapeutic target for bladder carcinoma treatment.
