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Updated: Dec 14, 2025
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
Published on: July 12, 2017
Generation of Knockout Gene-Edited Human Intestinal Organoids
Chathruckan Rajendra1,2, Tomas Wald2, Kevin Barber3,4
1Department of Pediatrics, University of California, San Francisco, San Francisco, CA, USA.
Abstract:
We discuss a methodology to generate and study knockout gene-edited human intestinal organoids. We describe the generation of knockout human embryonic stem cell lines that we then differentiate into mature human intestinal organoid tissue in Matrigel using several growth factors. We also discuss a pair of assays that can be used to study the integrity of the intestinal epithelial barrier of the human intestinal organoids under inflammatory stress conditions.
