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Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer
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Modeling Colorectal Cancer Progression Through Orthotopic Implantation of Organoids
Felipe de Sousa E Melo1, Jonathan M Harnoss2, Noelyn Kljavin3
1Discovery Oncology, Genentech, Inc., South San Francisco, CA, USA. desousaf@gene.com.
Methods in Molecular Biology (Clifton, N.J.)
|July 25, 2020
Summary
New methods allow researchers to study colorectal cancer (CRC) metastasis in mice. These models help understand how CRC spreads and evaluate new treatments for metastatic disease.
Area of Science:
- Oncology
- Cancer Biology
- Translational Research
Background:
- Colorectal cancer (CRC) mortality is often linked to metastatic disease.
- Existing mouse models have limitations in recapitulating CRC metastasis.
- Developing relevant in vivo models is crucial for preclinical therapeutic evaluation.
Purpose of the Study:
- To establish novel methodologies for studying colorectal cancer progression and metastasis in vivo.
- To create more accurate preclinical models for evaluating treatments targeting metastatic CRC.
Main Methods:
- Injection of CRC cell lines and organoids directly into the colorectal mucosa of mice.
- Surgical procedure for direct injection of CRC cells into the portal venous circulation.
Main Results:
- Developed orthotopic models that allow for spontaneous metastasis to the liver.
- Established liver metastasis models independent of a primary tumor, enabling focused study.
Conclusions:
- The described methodologies provide robust in vivo models for CRC metastasis research.
- These models facilitate a deeper understanding of metastatic mechanisms and preclinical drug testing for CRC.
