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The quest of cell surface markers for stem cell therapy
Anna Meyfour1,2, Sara Pahlavan2, Mehdi Mirzaei3,4
1Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cellular and Molecular Life Sciences : CMLS
|July 26, 2020
Summary
Identifying specific cell surface markers is crucial for stem cell therapies. New proteomics methods, including single-cell proteomics, enhance the purification of stem cells for regenerative medicine applications.
Area of Science:
- Biochemistry
- Cell Biology
- Regenerative Medicine
Background:
- Stem cells offer potential for tissue replacement therapies but face challenges due to culture heterogeneity.
- Targeting cell surface proteins with antibodies is key for isolating specific stem cell populations.
- Reliable cell surface markers are needed for research and clinical applications.
Purpose of the Study:
- To review methodologies for discovering cell surface markers.
- To introduce single-cell proteomics as an advanced tool for marker discovery.
- To discuss existing markers for stem cells and their lineages, focusing on nervous system, heart, pancreas, and liver.
Main Methods:
- Review of existing methodologies for cell surface marker discovery.
- Introduction of single-cell proteomics techniques.
- Analysis of proteomics-derived data.
Main Results:
- Proteomics has significantly aided in identifying cell surface markers, including plasma membrane proteins, ion channels, transporters, and signaling molecules.
- Specific cell surface markers for stem cells in the nervous system, heart, pancreas, and liver are discussed.
- Gaps in marker discovery and the potential of single-cell proteomics are highlighted.
Conclusions:
- Cell surface markers are vital for purifying specific stem cell types and stages.
- Single-cell proteomics offers advanced capabilities for discovering novel markers.
- Further research into surface markers, particularly for progenitor cells, could advance regenerative medicine.
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