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RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

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Pre-mRNA Processing: Modification of pre-mRNA Ends

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Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...

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Related Experiment Video

Updated: Dec 13, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

Proteome Diversification by RNA Editing.

Eli Eisenberg¹

¹Raymond and Beverly Sackler School of Physics and Astronomy and Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, Israel. elieis@post.tau.ac.il.

Methods in Molecular Biology (Clifton, N.J.)

|July 31, 2020

Summary

This summary is machine-generated.

RNA editing, particularly A-to-I changes by ADAR enzymes, creates protein diversity. Thousands of recoding sites are found across species, highlighting their evolutionary significance.

Keywords:

ADAR RNA editing Recoding

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Area of Science:

Molecular Biology
Genomics
Evolutionary Biology

Background:

RNA editing modifies RNA sequences, diverging from the genomic blueprint.
Adenosine to Inosine (A-to-I) editing, mediated by ADAR enzymes, is the most prevalent form.
Recoding sites within RNA can lead to altered protein products, increasing proteomic complexity.

Purpose of the Study:

To describe the phenomenon of RNA recoding across different species.
To explore the role of recoding in biological diversity and adaptation.
To discuss the evolutionary aspects of RNA recoding.

Main Methods:

Computational transcriptomic analyses to identify recoding sites.
Comparative genomics to assess conservation of recoding sites across lineages.
Literature review and synthesis of existing research on RNA editing and evolution.

Main Results:

Thousands of recoding sites have been identified in multiple species.
Many recoding sites are conserved within species lineages but not typically across them.
These sites possess functional and evolutionary importance.

Conclusions:

RNA recoding significantly contributes to proteome complexity and diversification.
Recoding sites play a crucial role in species adaptation and evolution.
Understanding recoding mechanisms is key to comprehending genome evolution and biological novelty.