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Using PC/GENE for protein and nucleic acid analysis
J Moore1, A Engelberg, A Bairoch
1Intelli-Genetics, Mountain View, CA 94040.
Biotechniques
|June 1, 1988
Summary
This study details protein analysis using PC/GENE software, translating nucleic acid sequences into amino acids to predict protein properties and structures. It enables sequence comparison and database searching for biological insights.
Area of Science:
- Molecular Biology
- Bioinformatics
- Computational Biology
Background:
- Protein analysis is crucial for understanding biological functions.
- Sequence analysis software aids in predicting protein characteristics and relationships.
Purpose of the Study:
- To describe protein analysis using the PC/GENE software package.
- To demonstrate the software's capabilities in sequence translation and property prediction.
Main Methods:
- Nucleic acid sequence editing and translation into amino acid sequences.
- Determination of amino acid composition, isoelectric point, and molecular weight.
- Prediction of secondary structure, membrane associations, hydrophobic/hydrophilic regions, and antigenic sites.
- Database searching for similar sequences and performing sequence alignments.
Main Results:
- The PC/GENE software facilitates comprehensive protein analysis from nucleic acid sequences.
- The study successfully predicted various protein properties and structural features.
- Database searches enabled identification of sequence similarities.
Conclusions:
- PC/GENE is a valuable tool for molecular biologists and bioinformaticians.
- The software supports detailed protein characterization and comparative sequence analysis.