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Microsphere-assisted super-resolved Mueller matrix microscopy.

Vahid Abbasian, Ali-Reza Moradi

|August 1, 2020

Summary

This summary is machine-generated.

Microsphere-assisted Mueller matrix microscopy (MMM) enhances imaging resolution beyond the diffraction limit. This technique improves microstructural and optical analysis of specimens, offering high-resolution polarimetric imaging capabilities.

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Area of Science:

Optical microscopy
Polarimetry
Materials science

Background:

Mueller matrix microscopy (MMM) provides detailed microstructural and optical information.
Achieving high lateral resolution is a significant challenge in MMM.

Purpose of the Study:

To enhance the lateral resolution of Mueller matrix microscopy.
To develop a method for resolution-enhanced polarimetric imaging.

Main Methods:

Integration of a transparent microsphere (MS) into the optical path of an MMM system.
Positioning the MS near the sample within the microscope objective's working distance.
Experimental validation using a holographic diffraction grating beyond the diffraction limit of a 10× objective.

Main Results:

The microsphere-assisted MMM system demonstrated increased resolution, surpassing the diffraction limit.
Higher spatial frequencies from the sample were redirected into the microscope's acceptance cone by the MS.
Two-dimensional Mueller matrix images and quantitative polarimetric parameters were successfully acquired and compared.

Conclusions:

Microsphere insertion is an effective method to achieve resolution-enhanced polarimetric imaging in MMM.
The proposed technique is easily implemented and offers potential for high-resolution visualization of microscopic object polarization characteristics.