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Quantitative ciprofloxacin on-site rapid detections using quantum dot microsphere based immunochromatographic test
Jing Liu1, Bin Wang1, Huachuan Huang2
1Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education & Single Molecule Nanometry Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Food Chemistry
|August 4, 2020
Summary
We developed a novel quantum dot microsphere (QDM) immunochromatographic test strip for rapid and accurate ciprofloxacin (CIP) detection. This sensitive method offers a low detection limit and a wide linear range for quantitative analysis.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Materials Science
Background:
- Ciprofloxacin (CIP) abuse poses significant risks to human health.
- Accurate and rapid detection methods for CIP are crucial for monitoring and control.
Purpose of the Study:
- To design and develop a quantum dot microsphere (QDM) based immunochromatographic quantitative test strip for CIP detection.
- To evaluate the sensitivity, accuracy, selectivity, reproducibility, and stability of the developed test strip.
- To fabricate a portable, smartphone-based reader for automated CIP quantification.
Main Methods:
- Utilized quantum dot microspheres (QDMs) conjugated with monoclonal antibodies (mAbs) for CIP detection.
- Employed an immunochromatographic assay principle where reduced fluorescence intensity indicates CIP presence.
- Developed a 3-D printed smartphone reader for automated quantitative analysis.
Main Results:
- Achieved a low detection limit of 0.05 ng/mL for CIP.
- Established a wide linear detection range from 0.1 ng/mL to 100 ng/mL.
- Demonstrated high sensitivity, accuracy, selectivity, reproducibility, and stability.
- The entire detection process takes 15 minutes and costs approximately 1 RMB.
Conclusions:
- The QDM-based immunochromatographic test strip provides a highly sensitive, accurate, and cost-effective method for quantitative CIP detection.
- The integrated smartphone reader enables rapid, automated, and portable analysis.
- This technology holds promise for effective monitoring of CIP contamination in various samples.
