Relationship of Structural and Tissue Components of Full-Layer Skin Wound and Mathematical Modeling of the Healing...

We studied the relationship between structural components of the wound (scab, leukocyte shaft, newly formed epithelium, and granulation tissue) and tissue components (epithelial and connective tissue) during spontaneous healing of skin wounds in rats. The regenerate structures were evaluated by microscopic morphometry. The number of vessels in 10 fields of view was calculated (at ×600), the dimensions of newly formed regenerate structures were determined: height of the scab, leukocyte shaft, granulation tissue, the border zone of the epithelium, and the length of the epithelial wedge. For the studied morphometric parameters, regression models of changes in the parameters of the regenerating wound were created depending on the term of the reparative process (days 7 and 14). The correlation analysis of the morphometric parameters of the regenerating wound revealed bilateral strong (direct and inverse) correlations in pairs. Most of the identified dependencies were linear and the changes in the number of vessels in the granulation tissue fit the quadratic regression law. The regularities observed by us during spontaneous healing of full-layer skin defects can be considered as control values for comparison with reparation of similar wounds under conditions of stimulation.