Related Experiment Video
Updated: Dec 13, 2025
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method
Published on: October 7, 2025
Catalytic hairpin assembly-based electrochemical biosensor with tandem signal amplification for sensitive microRNA
Lin Cui1, Jinghua Zhou1, Xiao-Yun Yang2
1College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China. cyzhang@sdnu.edu.cn wangxl@sdnu.edu.cn.
Abstract:
We demonstrate for the first time the construction of a low background electrochemical biosensor with tandem signal amplification for sensitive microRNA assay based on target-activated catalytic hairpin assembly (CHA) of heteroduplex-templated copper nanoparticles. This electrochemical biosensor exhibits high sensitivity, good specificity, single-base mismatch discrimination capability, excellent stability and reproducibility, and it can sensitively detect microRNA in cancer cells.
