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Updated: Dec 12, 2025

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

Superresolution Microscopy of Sphingolipids.

Jan Schlegel¹, Markus Sauer²

¹Department of Biotechnology and Biophysics, Biocenter, University of Wuerzburg, Wuerzburg, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|August 10, 2020

Summary

This summary is machine-generated.

This chapter details labeling sphingolipids for superresolution microscopy, specifically using direct stochastic optical reconstruction microscopy (dSTORM). It covers custom fluorophore conjugation and sample preparation for enhanced visualization.

Keywords:

Sphingolipids Superresolution microscopy dSTORM

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Last Updated: Dec 12, 2025

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

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Published on: October 15, 2016

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Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer

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Published on: February 11, 2015

Area of Science:

Cell Biology
Biophysics
Microscopy

Background:

Sphingolipids are crucial membrane components involved in various cellular processes.
Visualizing sphingolipids at high resolution is essential for understanding their function.
Superresolution microscopy offers unprecedented detail in biological imaging.

Purpose of the Study:

To provide a detailed protocol for labeling and visualizing sphingolipids.
To focus on the application of direct stochastic optical reconstruction microscopy (dSTORM).
To enable high-resolution imaging of sphingolipid distribution and dynamics.

Main Methods:

Custom fluorophore conjugation to sphingolipid-binding molecules (e.g., toxins, antibodies).
Development of a step-by-step labeling protocol for biological samples.
Application of superresolution microscopy techniques, particularly dSTORM.

Main Results:

Successful labeling and visualization of sphingolipids using the developed protocol.
Demonstration of dSTORM for high-resolution imaging of sphingolipid localization.
Insights into sphingolipid organization within cellular membranes.

Conclusions:

The provided protocol enables effective sphingolipid labeling for superresolution microscopy.
dSTORM is a powerful technique for studying sphingolipid behavior at the nanoscale.
This method facilitates further research into the biological roles of sphingolipids.