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Martin Sidler^1,2,3,4, Karen J Aitken¹, Sarah Forward⁵

¹The Hospital for Sick Children, Research Institute, Developmental and Stem Cell Biology, Toronto, Canada.

Bladder (San Francisco, Calif.)

|August 11, 2020

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A new non-invasive method using continuous urine weight recording reliably assesses mouse bladder function. This technique is ideal for long-term studies, unlike invasive methods that can alter results.

Area of Science:

Urology
Animal Models
Physiology

Background:

Assessing murine bladder function is crucial for understanding urological conditions.
Invasive methods can compromise histological and genetic analyses.
A reliable, non-invasive technique is needed for long-term monitoring.

Purpose of the Study:

To review existing methods for assessing rodent bladder function.
To evaluate a novel non-invasive technique for long-term murine bladder function recording.

Main Methods:

Literature review of rodent bladder function assessment techniques.
Utilized modified metabolic cages for continuous urine weight recording in mice.
Pilot study involved female mice with partial bladder outlet obstruction or sham operations, with some receiving an S6K-inhibitor treatment.

Keywords:

S6K VSOP animal model bladder hyperactivity bladder physiology cystometry murine micturition assessment partial bladder outlet obstruction

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Main Results:

Continuous urine weight recording successfully detected changes in murine bladder function.
Observed a hyperactive voiding pattern associated with both partial bladder outlet obstruction and S6K-inhibitor treatment.
The non-invasive method proved reliable in detecting functional alterations.

Conclusions:

Continuous urine weight recording is a reliable, cost-effective, and non-invasive method for assessing murine bladder function.
This technique is suitable for long-term applications.
It avoids the histological and genetic analysis limitations of invasive methods.