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Related Experiment Video

Updated: Dec 12, 2025

Author Spotlight: Innovative Microneedle-Based Strategies for Enhanced Exosome Delivery and Stability
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Biodegradable microneedle patch for transdermal gene delivery.

Moyuan Qu1, Han-Jun Kim, Xingwu Zhou

  • 1Department of Bioengineering, Center for Minimally Invasive Therapeutics, California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA. khademh@terasaki.org wsun@terasaki.org.

Nanoscale
|August 14, 2020
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microneedle patch for effective transdermal delivery of plasmid DNA (pDNA) using nanoparticles. This platform shows promise for treating skin conditions and cancer through controlled nucleic acid delivery.

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Area of Science:

  • Biomaterials Science
  • Dermatology
  • Gene Therapy

Background:

  • The skin's complex immune and vascular systems present challenges for effective drug delivery.
  • Nucleic acid delivery to skin holds potential for treating various dermatological conditions, including cancer and genetic disorders.
  • Existing methods often lack efficiency and control for localized nucleic acid delivery.

Purpose of the Study:

  • To develop and evaluate a gelatin methacryloyl (GelMA) microneedle (MN) platform for transdermal plasmid DNA (pDNA) delivery.
  • To investigate the use of poly(β-amino ester) (PBAE) nanoparticles for efficient intracellular nucleic acid delivery.
  • To demonstrate controlled and sustained release of pDNA from the microneedle system.

Main Methods:

  • Fabrication of GelMA microneedles loaded with PBAE/pDNA nanoparticles.
  • In vitro and in vivo assessment of transfection efficiency and pDNA release kinetics.
  • Modulation of pDNA release profiles by adjusting the crosslinking degree of the GelMA hydrogel.

Main Results:

  • High in vitro and in vivo transfection efficiency achieved with the GelMA MN platform.
  • Sustained release of pDNA-encapsulated PBAE nanoparticles from the microneedles.
  • Tunable release profiles demonstrated by altering GelMA hydrogel crosslinking.

Conclusions:

  • The developed PBAE/DNA NP-embedded GelMA MN patches offer a promising strategy for localized and controlled transdermal pDNA delivery.
  • This platform has significant potential for applications in tissue regeneration and cancer therapy.
  • The system provides a controllable method for nucleic acid delivery, overcoming previous limitations.