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Reflections on the OECD guidelines for in vitro skin absorption studies
N B Hopf1, C Champmartin2, L Schenk3
1Centre for Primary Care and Public Health (Unisante), Department for Occupational and Environmental Health (DSTE), Exposure Science Unit, Switzerland.
Regulatory Toxicology and Pharmacology : RTP
|August 14, 2020
Summary
Researchers discussed in vitro skin permeability experiments, identifying gaps in OECD guidelines for skin absorption studies. They propose improvements to harmonize future testing and enhance accuracy in measuring chemical exposure on skin.
Area of Science:
- Toxicology and Environmental Health
- Dermal Absorption and Toxicology
Background:
- The 8th Occupational and Environmental Exposure of the Skin to Chemicals (OEESC) conference convened researchers to discuss in vitro skin permeability assays.
- Focus was placed on existing OECD guidelines for skin absorption, specifically Guidance Document 28, Test Guideline 428, and Guidance Notes 156.
Purpose of the Study:
- To address concerns that current OECD guidance documents inadequately cover methodological issues and test performance in skin permeation studies.
- To identify factors influencing skin permeation and its measurement that are often omitted or undiscussed in published research.
Main Methods:
- Discussions among hands-on skin permeation researchers during the OEESC conference.
- Review and critical analysis of OECD Guidance Document 28, Test Guideline 428, and OECD Guidance Notes 156.
Main Results:
- A consensus emerged that existing OECD guidelines lack comprehensive details on methodological aspects and test performance for in vitro skin absorption studies.
- Numerous factors influencing skin permeation and its measurement were identified, with guidance often missing in current documents and literature.
Conclusions:
- Current OECD guidance documents for in vitro skin permeation studies require significant updates to address methodological complexities.
- Proposed improvements to these guidelines are essential for harmonizing future experiments and ensuring reliable dermal absorption data.
- Enhanced guidance will improve the accuracy and consistency of in vitro skin permeability measurements in chemical safety assessments.
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