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On-line sensors for coagulation proteins: concept and progress report
J D Andrade1, J Herron, J N Lin
1Department of Bioengineering, College of Engineering, University of Utah, Salt Lake City 84112.
Biomaterials
|January 1, 1988
Summary
This study introduces novel fluoroimmunoassay-based immunosensors for real-time monitoring of blood damage and coagulation. These sensors aim to improve the assessment of cardiovascular device interactions with blood systems.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Clinical Diagnostics
Background:
- Assessing blood damage and system activation by medical devices is challenging.
- Immunoassays can measure proteins involved in coagulation, complement, and inflammation.
Purpose of the Study:
- To develop remote, on-line, semicontinuous immunosensors for coagulation proteins.
- To create a system for monitoring blood-device interactions.
Main Methods:
- Utilizing fluoroimmunoassay principles with optical free/bound separation.
- Employing evanescent wave excitation via total internal reflection and waveguide optics.
- Collecting fluorescence emission in the near field.
Main Results:
- Discusses methods for reagent delivery and antigen-antibody binding constant modification.
- Presents findings on non-specific binding and plasma-blood fluorescence.
- Evaluates blood compatibility aspects of the developed system.
Conclusions:
- The proposed immunosensor array offers a promising approach for continuous monitoring of blood-device interactions.
- This technology could enhance the safety and efficacy of cardiovascular and extracorporeal devices.