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Functional Omics Identifies Serine Hydrolases That Mobilize Storage Lipids during Rice Seed Germination.
Achintya Kumar Dolui1,2, Panneerselvam Vijayaraj3,2
1Lipid and Nutrition Laboratory, Department of Lipid Science, Council of Scientific and Industrial Research-Central Food Technological Research Institute, Mysore, Karnataka, 570020, India.
This study identifies active lipases and other Serine Hydrolases (SHs) in germinating rice seeds using activity-based protein profiling. These enzymes are crucial for lipid mobilization and seedling growth, with their activity impacted by fungal infection.
Area of Science:
- Plant biochemistry
- Proteomics
- Lipidomics
Background:
- Understanding proteolipidome dynamics is key to plant physiology and disease.
- Protein function relies on post-translational modifications beyond sequence annotation.
Purpose of the Study:
- To identify active lipases and Serine Hydrolases (SHs) during rice seed germination.
- To correlate enzyme activity with lipid metabolism and seedling development.
- To investigate the role of SHs in plant response to biotic stress.
Main Methods:
- Gel-free activity-based protein profiling to map active SHs.
- Microarray analysis to monitor gene expression of identified SHs.
- Shotgun lipidome analysis to study lipid molecular species distribution.
Main Results:
- 43 active SHs, including lipases, proteases, and esterases, were identified.
- Distinct lipidome patterns and metabolic connections to seedling growth were revealed.
- Lipase activity and lipid mobilization were time-dependently correlated with SH expression and impacted by fungal infection.
Conclusions:
- Functional proteome and lipidome strategies effectively identify active SHs.
- Lipid homeostasis regulated by SHs is vital for rice seed germination and growth.
- SHs play a significant role in rice seedling response to Fusarium infection.
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