Related Experiment Video
Updated: Dec 11, 2025
10:17
An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
Published on: November 3, 2010
23.2K
A report on DNA sequence determinants in gene expression
Ravail Singh1, Yengkhom Sophiarani2
1Indian Institute of Integrative Medicine, CSIR, Canal Road, Jammu-180001.
Bioinformation
|August 25, 2020
Summary
Gene expression is influenced by nucleotide bias in coding sequences. This review examines DNA sequence elements and constraints affecting protein production and gene expression rates.
Area of Science:
- Molecular Biology
- Genetics
- Bioinformatics
Background:
- Nucleotide usage bias in coding sequences correlates with gene expression levels.
- Protein production is determined by complex interactions within the gene expression system.
- Polypeptide chain elongation rate is influenced by codon usage bias and amino acid composition.
Purpose of the Study:
- To review local DNA sequence elements impacting gene expression.
- To investigate positional and combinatorial constraints in gene expression.
Main Methods:
- Literature review of studies on codon usage bias and gene expression.
- Analysis of DNA sequence elements and their role in protein synthesis.
- Examination of constraints affecting polypeptide chain elongation.
Main Results:
- Codon usage bias significantly impacts gene expression efficiency.
- Specific nucleotide sequences and amino acid compositions influence protein production.
- Positional and combinatorial constraints play a crucial role in regulating gene expression.
Conclusions:
- Understanding nucleotide bias and sequence constraints is key to deciphering gene expression regulation.
- Further research into these elements can optimize protein production in synthetic biology.
- This review consolidates knowledge on factors governing gene expression.
More Related Videos
Related Concept Videos
02:11Reporter Genes
12.6K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
12.6K
02:02Cis-regulatory Sequences
11.4K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.4K
02:02Cis-regulatory Sequences
3.8K
3.8K
02:24Ribosome Profiling
4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K
01:36What is Gene Expression?
10.4K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
10.4K
01:42What is Gene Expression?
189.2K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
189.2K