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Related Experiment Videos

Surface immunoglobulins on stimulated lymphocytes.

I Trebichavský, L Jarosková, M Pospísil

    Folia Biologica
    |January 1, 1977
    PubMed
    Summary

    Newborn piglet spleen cells express only IgM surface immunoglobulin, which increases with mitogen stimulation. This study investigates immune responses in young swine lymphocytes.

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    Area of Science:

    • Immunology
    • Veterinary Science
    • Swine Research

    Background:

    • Surface immunoglobulins (Ig) are crucial for lymphocyte function.
    • Understanding lymphocyte responses in newborn animals is vital for immunology and veterinary medicine.
    • Piglets possess unique immune system development characteristics.

    Purpose of the Study:

    • To investigate the types of surface immunoglobulins on newborn piglet spleen lymphocytes.
    • To determine the effect of various mitogens on immunoglobulin expression.
    • To characterize the early immune cell responses in piglets.

    Main Methods:

    • Lymphocytes were isolated from newborn piglet spleens.
    • Cells were cultured with phytohaemagglutinin, lipopolysaccharide (LPS), lipid A, and streptococcal erythrogenic toxin.
    • Surface immunoglobulin expression was analyzed using iodinated anti-pig Ig, mu-, and gamma-antisera.

    Main Results:

    • Only IgM was identified as the surface immunoglobulin on both non-stimulated and LPS-stimulated piglet lymphocytes.
    • Cultivation with all tested mitogens resulted in an increased number of labelled cells, indicating heightened lymphocyte activity.
    • Specific responses to different mitogens suggest varied activation pathways.

    Conclusions:

    • Newborn piglet spleen lymphocytes predominantly express IgM.
    • Mitogenic stimulation enhances the expression or accessibility of surface IgM on piglet lymphocytes.
    • These findings provide insights into the ontogeny of the porcine immune system.

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