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Changes in skeletal muscle diffusion parameters owing to intramyocellular lipid.

Kiichi Tadano1, Yoshikazu Okamoto2, Tomonori Isobe2

  • 1Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan; Faculty of Health Sciences, Kyorin University, 5-4-1 Shimorenjaku, Mitaka, Tokyo 181-8612, Japan.

Magnetic Resonance Imaging
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Summary

Intramyocellular lipids (IMCL) influence water diffusion in skeletal muscle cells of patients with lifestyle-related diseases. Higher IMCL amounts correlate with reduced diffusion, suggesting IMCL acts as a diffusion-limiting factor.

Keywords:
(1)H-MRSDTIIntramyocellular lipidSkeletal muscle

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Area of Science:

  • Biophysics
  • Skeletal Muscle Physiology
  • Medical Imaging

Background:

  • Hybrid training (HYBT) increases diffusion in skeletal muscle cells.
  • This increase is attributed to enlarged intracellular space from muscle hypertrophy.
  • A hypothesis suggests reduced intramyocellular lipids (IMCL) also contribute to this diffusion change.

Purpose of the Study:

  • To investigate whether intramyocellular lipids (IMCL) act as a diffusion-limiting factor for water molecules in skeletal muscle cells.

Main Methods:

  • Quantified IMCL in triceps surae muscles of patients and healthy volunteers.
  • Measured apparent diffusion coefficient (ADC), diffusion anisotropy (FA), and diffusion eigenvalues (λ1, λ2, λ3).
  • Correlated IMCL levels with diffusion parameters.

Main Results:

  • In patients, IMCL positively correlated with FA and negatively with λ3.
  • Weak negative correlations were found between IMCL and ADC, λ1, and λ2.
  • No correlation was observed in healthy volunteers.

Conclusions:

  • Intramyocellular lipids (IMCL) can influence water molecule diffusion in skeletal muscle cells, particularly at higher concentrations.
  • IMCL acts as a diffusion-limiting factor, though its effect may be less pronounced than that of cell membranes.