Related Experiment Video
Updated: Dec 10, 2025
08:41
Mouse Round Spermatid Injection
Published on: January 26, 2024
1.2K
Reverse Transcriptase Affects Gametogenesis and Preimplantation Development in Mouse.
Chrysoula Kitsou1, Leandros Lazaros1,2, Alexandra Papoudou-Bai3
1Laboratory of Medical Genetics of Human Reproduction, Medical School, Ioannina University, Ioannina, Greece.
In Vivo (Athens, Greece)
|September 3, 2020
Summary
Lamivudine, a reverse transcriptase inhibitor, negatively impacts male and female reproductive functions and early mouse embryo development. This study reveals its detrimental effects on gametogenesis and preimplantation embryo growth.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Pharmacology
Background:
- Reverse transcriptase (RT) is crucial for cell growth and differentiation, found in ovaries, testes, gametes, and embryos.
- Lamivudine is a potent inhibitor of reverse transcriptase.
Purpose of the Study:
- To investigate the effects of lamivudine on gametogenesis and preimplantation mouse embryo development.
- To assess lamivudine's impact on reproductive parameters and early embryonic growth.
Main Methods:
- Male and female FVB/N mice were administered lamivudine for seven weeks.
- Evaluated sperm parameters, testicular and ovarian morphology, and in vitro fertilization (IVF) embryo development post-treatment.
Main Results:
- Lamivudine treatment impaired sperm parameters and testicular structure in males.
- Ovarian morphology showed reduced primordial germ cells and primary oocytes in females.
- Embryo development was hindered, affecting morphology and blastocyst formation during in vitro culture.
Conclusions:
- Lamivudine administration adversely affects spermatogenesis and oogenesis.
- The study demonstrates lamivudine's negative impact on in vitro preimplantation mouse embryo development.
