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Published on: June 2, 2015
An Improved Encapsulation Method for Cryopreserving Hepatocytes for Functional Transplantation Using a
Kosuke Yamada1, Takeshi Aoki2, Yuta Enami1
1Department of Gastroenterological and General Surgery, School of Medicine, Showa University, Tokyo, Japan.
Background/Aim:
Thermo-reversible gelation polymer (TGP) can be converted into a gel state upon warming and liquid upon cooling. The present study aimed to demonstrate a new method for cryopreservation and encapsulation of rat hepatocytes using a TGP and their successful transplantation.
Materials And Methods:
The isolated rat hepatocytes were microencapsulated using TGP, and stored in liquid nitrogen. After cryopreservation, hepatocytes were cultured. Moreover, hepatocytes were transplanted into the spleen without a TGP capsule.
Results:
The viability of hepatocytes that were cryopreserved in TGP was 71.2±2.3%. The hepatocytes demonstrated adequate survival, maintained their hepatic function in culture, and expressed albumin after transplantation to the rat spleen.
Conclusion:
We demonstrated a cryopreservation method of rat hepatocyte encapsulation using a TGP gel in the hydrogel state which subsequently allowed successful transplantation of unencapsulated hepatocytes in a sol state TGP gel at low temperature.
