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Actin networks regulate the cell membrane permeability during electroporation.

Aswin Muralidharan1, Lea Rems2, Michiel T Kreutzer1

  • 1Department of Chemical Engineering, Delft University of Technology, van der Maasweg 9, 2629 HZ Delft, the Netherlands.

Biochimica Et Biophysica Acta. Biomembranes
|September 4, 2020
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Summary

Disrupting cell actin networks enhances molecule delivery via electroporation by increasing membrane permeability. This temperature-dependent effect suggests actin

Keywords:
Actin networksElectropermeabilizationElectroporationEnergy barrierTemperature dependent kinetics of electroporation

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Area of Science:

  • Cell Biology
  • Biophysics

Background:

  • Electroporation uses electric pulses to temporarily disrupt cell membranes for molecule delivery.
  • Cell membrane permeability is crucial for efficient exogenous molecule uptake.

Purpose of the Study:

  • To investigate the role of actin networks in regulating cell membrane permeability during electroporation.
  • To determine how actin disruption affects molecular uptake and the energy barrier of electroporation.

Main Methods:

  • Electroporation of cells at varying temperatures (11°C to 37°C).
  • Quantification of membrane-impermeable molecule (propidium iodide) uptake.
  • Analysis of temperature-dependent uptake kinetics.
  • Numerical calculations of transmembrane voltage.

Main Results:

  • Disruption of actin networks significantly increased the uptake of propidium iodide during electroporation.
  • Molecular uptake during electroporation was observed to increase with temperature.
  • Disrupting actin networks lowered the activation energy barrier for electroporation.
  • The reduced energy barrier was attributed to changes in membrane mechanical properties, not transmembrane voltage.

Conclusions:

  • Actin networks play a regulatory role in cell membrane permeability during electroporation.
  • Disrupting actin networks enhances the efficiency of delivering exogenous molecules into cells.
  • Current electroporation models should incorporate cytoskeletal contributions to membrane permeability for improved accuracy.